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货号： bs-0481R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0481R
- 英文名称
- IFN gamma
- 中文名称
- 干扰素-γ/IFN-γ抗体
- 别 名
- IFNG; IFG; IFI; IFN Gamma; IFN Immune; IFN-gamma; IFNG; IFNG_HUMAN; Immune Interferon; Interferon gamma; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon.
- Specific References (4) | bs-0481R has been referenced in 4 publications.[IF=5.52] Nimmagadda, Vamshi K., et al. "Overexpression of SIRT1 Protein in Neurons Protects against Experimental Autoimmune Encephalomyelitis through Activation of Multiple SIRT1 Targets." The Journal of Immunology (2013). Mouse.PubMed:23547115[IF=2.00] Wang, Lu, et al. "Deficiency of Interferon-Gamma or Its Receptor Promotes Colorectal Cancer Development." Journal of Interferon & Cytokine Research35.4 (2015): 273-280. WB ; Mouse.PubMed:25383957[IF=1.64] Guo, Jie, Jiang Qian, and Rui Zhang. "Histopathology and immunohistochemical profile in idiopathic dacryoadenitis." Current Eye Research 37.5 (2012): 365-371. IHC-P ; Human.PubMed:22510006[IF=2.13] Wang, Lu, et al. "Deficiency of interferon-gamma or its receptor promotes colorectal cancer development." Journal of Interferon & Cytokine Research 35.4 (2015): 273-280. WB ; Mouse.PubMed:25383957
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 免疫学 信号转导 干扰素
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 15kDa
- 细胞定位
- 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human IFN Gamma:81-166/166
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
Function:
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Subunit:
Homodimer.
Subcellular Location:
Secreted.
Tissue Specificity:
Released primarily from activated T lymphocytes.
Post-translational modifications:
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
DISEASE:
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Similarity:
Belongs to the type II (or gamma) interferon family.
SWISS:
P01579
Gene ID:
3458
Database links:Entrez Gene: 3458 Human
Entrez Gene: 15978 Mouse
Entrez Gene: 360697 Rat
Omim: 147570 Human
SwissProt: P01579 Human
SwissProt: P01580 Mouse
Unigene: 856 Human
Unigene: 240327 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
HL-60 Cell (Human) Lysate at 40 ug
Primary: Anti- IFN gamma (bs-0481R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 16 kD
Sample:
Molt-4 Cell (Human) Lysate at 40 ug
Primary: Anti- IFN gamma (bs-0481R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 16 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IFN gamma Polyclonal Antibody, Unconjugated(bs-0481R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining