| 货号 | MAB1440-SP |
| 别名 | GLUT2 Glucose transporter type 2, liver; GLUT-2; SLC2A2; solute carrier family 2 (facilitated glucose transporter), member 2; solute carrier family 2, facilitated glucose transporter member 2 | 全称 | Glucose Transporter Type 2 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects mouse Glut2. Recognizes mouse Glut2-transfected NS0 cells but not NS0 control transfectants. It also detects Glut2 on mouse insulinoma beta TC-6 cells (2). |
| 使用方法 | Flow Cytometry: 2.5 µg/106cells Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6514 (Human); 20526 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cholesterol Elevation Impairs Glucose-Stimulated Ca2+ Signaling in Mouse Pancreatic beta-Cells. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | CHO Chinese hamster ovary cell line transfected with mouse Glut2 Met1-Val523 Accession # P14246 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Glut2 in beta TC‑6 Mouse Cell Line. Glut2 was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Rat Anti-Mouse Glut2 Monoclonal Antibody (Catalog # MAB1440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
