Human alpha-Smooth Muscle Actin MAb (Clone 1A4) (25 UG)【科瑞奥博】

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Human alpha-Smooth Muscle Actin MAb (Clone 1A4) (25 UG)

货号： MAB1420-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1420-SP
别名	AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin
全称	Actin, Alpha 2, Smooth Muscle, Aorta
反应种属	Human
应用	Western Blot,Simple Western,Immunohistochemistry,CyTOF-ready,Immunocytochemistry,Intracellular Staining by Flow Cytometry
目标/特异性	Detects human alpha -Smooth Muscle Actin.
使用方法	Western Blot: 0.25 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 8-25 µg/mL Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	59 (Human); 11475 (Mouse); 81633 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Exosomes in Human Breast Milk Promote EMT Authors: W Qin, Y Tsukasaki, S Dasgupta, N Mukhopadhy, M Ikebe, ER Sauter Clin Cancer Res, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: IF Generation of Induced Cardiospheres via Reprogramming of Skin Fibroblasts for Myocardial Regeneration Authors: Jian-Yong Xu Stem Cells, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: IHC - PFA fixed Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling. Authors: Riordan D, Varma S, West R, Brown P PLoS ONE, 2015;10(7):e0128975. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Association between PON1 rs662 polymorphism and coronary artery disease. Authors: Liu T, Zhang X, Zhang J, Liang Z, Cai W, Huang M, Yan C, Zhu Z, Han Y Eur J Clin Nutr, 2014;68(9):1029-35. Species: Human Sample Type: Whole Tissue Application: IHC - paraffin Occurrence of tertiary lymphoid tissue is associated with T-cell infiltration and predicts better prognosis in early-stage colorectal cancers. Authors: Di Caro G, Bergomas F, Grizzi F, Doni A, Bianchi P, Malesci A, Laghi L, Allavena P, Mantovani A, Marchesi F Clin Cancer Res, 2014;20(8):2147-58. Species: Human Sample Type: tissue Application: IHC Paraffin-embedded Transcriptional effects of 1,25 dihydroxyvitamin D(3) physiological and supra-physiological concentrations in breast cancer organotypic culture. Authors: Milani C, Katayama M, de Lyra E, Welsh J, Campos L, Brentani M, Maciel M, Roela R, del Valle P, Goes J, Nonogaki S, Tamura R, Folgueira M BMC Cancer, 2013;13(0):119. Species: Human Sample Type: whole cells Application: ICC Locally different endothelial nitric oxide synthase protein levels in ascending aortic aneurysms of bicuspid and tricuspid aortic valve. Authors: Mohamed SA, Radtke A, Saraei R Cardiol Res Pract, 2012;2012(0):165957. Species: Human Sample Type: Cell Lysates Application: WB The effect of VEGF on the myogenic differentiation of adipose tissue derived stem cells within thermosensitive hydrogel matrices. Authors: Kim MH, Hong HN, Hong JP, Park CJ, Kwon SW, Kim SH, Kang G, Kim M Biomaterials, 2010;31(6):1213-8. Species: Human Sample Type: Whole Cells Application: ICC Inhibition of caspase-3 differentially affects vascular smooth muscle cell apoptosis in the concave versus convex aortic sites in ascending aneurysms with a bicuspid aortic valve. Authors: Mohamed SA, Misfeld M, Hanke T, Charitos EI, Bullerdiek J, Belge G, Kuehnel W, Sievers HH Ann. Anat., 2010;192(3):145-50. Species: Human Sample Type: Whole Cells Application: ICC Lysosome-dependent Ca(2+) release response to Fas activation in coronary arterial myocytes through NAADP: evidence from CD38 gene knockouts. Authors: Zhang F, Xia M, Li PL Am. J. Physiol., Cell Physiol., 2010;298(5):C1209-16. Species: Mouse Sample Type: Whole Cells Application: ICC Effect of an antimicrobial agent on atherosclerotic plaques: assessment of metalloproteinase activity by molecular imaging. Authors: Ohshima S, Fujimoto S, Petrov A, Nakagami H, Haider N, Zhou J, Tahara N, Osako MK, Fujimoto A, Zhu J, Murohara T, Edwards DS, Narula N, Wong ND, Chandrashekhar Y, Morishita R, Narula J J. Am. Coll. Cardiol., 2010;55(12):1240-9. Species: Rabbit Sample Type: Whole Tissue Application: IHC Paraffin-embedded Hypoxia-induced alveolar epithelial-mesenchymal transition requires mitochondrial ROS and hypoxia-inducible factor 1. Authors: Zhou G, Dada LA, Wu M, Kelly A, Trejo H, Zhou Q, Varga J, Sznajder JI Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(6):L1120-30. Species: Rat Sample Type: Whole Cells Application: ICC Breast carcinoma-associated fibroblasts and their counterparts display neoplastic-specific changes. Authors: Hawsawi NM, Ghebeh H, Hendrayani SF, Tulbah A, Al-Eid M, Al-Tweigeri T, Ajarim D, Alaiya A, Dermime S, Aboussekhra A Cancer Res., 2008;68(8):2717-25. Species: Human Sample Type: Whole Tissue Application: IHC Molecular imaging of matrix metalloproteinase in atherosclerotic lesions: resolution with dietary modification and statin therapy. Authors: Fujimoto S, Hartung D, Ohshima S, Edwards DS, Zhou J, Yalamanchili P, Azure M, Fujimoto A, Isobe S, Matsumoto Y, Boersma H, Wong N, Yamazaki J, Narula N, Petrov A, Narula J J. Am. Coll. Cardiol., 2008;52(23):1847-57. Species: Rabbit Sample Type: Whole Tissue Application: IHC Gadodiamide contrast agent activates fibroblasts: a possible cause of nephrogenic systemic fibrosis. Authors: Edward M, Quinn JA, Mukherjee S, Jensen MB, Jardine AG, Mark PB, Burden AD J. Pathol., 2008;214(5):584-93. Species: Human Sample Type: Whole Cells Application: ICC Role of matrix metalloprotease-9 in hyperoxic injury in developing lung. Authors: Chetty A, Cao GJ, Severgnini M, Simon A, Warburton R, Nielsen HC Am. J. Physiol. Lung Cell Mol. Physiol., 2008;295(4):L584-92. Species: Mouse Sample Type: Tissue Homogenates Application: WB Differentiation of human circulating fibrocytes as mediated by transforming growth factor-beta and peroxisome proliferator-activated receptor gamma. Authors: Hong KM, Belperio JA, Keane MP, Burdick MD, Strieter RM J. Biol. Chem., 2007;282(31):22910-20. Species: Human Sample Type: Cell Lysates Application: WB Bronchoalveolar lavage fluid from patients with acute lung injury/acute respiratory distress syndrome induces myofibroblast differentiation. Authors: Synenki L, Chandel NS, Budinger GR, Donnelly HK, Topin J, Eisenbart J, Jovanovic B, Jain M Crit. Care Med., 2007;35(3):842-8. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Peptide corresponding toresidues EEEDSTALVC of the N-term of human alpha -smooth muscle actin (1)
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
运输条件	Blue Ice
存放说明	4℃
参考文献	Skalli, O. et al. (1986) J. Cell Biol. 103:2787. Oishi, K. et al. (2002) J. Physiol. 540:139.

参考图片

Detection of Human alpha ‑Smooth Muscle Actin by Western Blot. Western blot shows lysates of human heart tissue and iBJ6 human induced pluripotent stem cell line undifferentiated or cardiac differentiated. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human
alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha ‑Smooth Muscle Actin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

alpha ‑Smooth Muscle Actin in BG01V Human Embryonic Stem Cells. alpha ‑Smooth Muscle Actin was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiomyocytes using Mouse Anti-Human alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

alpha -Smooth Muscle Actin in Human Breast Cancer Tissue. alpha -Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human
alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Human
alpha ‑Smooth Muscle Actin by Simple Western^TM. Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyocytes (+), loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Smooth Muscle Actin at approximately 49 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.