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货号： bs-6252R-PE-Cy5 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-6252R-PE-Cy5
- 英文名称
- Anti-SIK1/PE-Cy5
- 中文名称
- PE-Cy5标记的丝氨酸/苏氨酸蛋白激酶SIK1抗体
- 别 名
- MSK; myocardial SNF1 like kinase; Salt inducible kinase 1; Salt-inducible protein kinase 1; Serine threonine protein kinase SNF1 like kinase 1; Serine threonine protein kinase SNF1LK; Serine/threonine-protein kinase SIK1; Serine/threonine-protein kinase SNF1-like kinase 1; Serine/threonine-protein kinase SNF1LK; SIK-1; SIK1; SIK1_HUMAN; SNF1 like kinase.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 肿瘤 细胞生物 免疫学 信号转导 细胞周期蛋白 转录调节因子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Horse,
- 产品应用
- IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 85kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Snf1lk
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
Transient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. Potential role in G2/M cell cycle regulation. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3.
Function:
Serine/threonine-protein kinase involved in variousprocesses such as cell cycle regulation, gluconeogenesis andlipogenesis regulation, muscle growth and differentiation and tumorsuppression. Phosphorylates HDAC4, HDAC5, PPME1, SREBF1,TORC1/CRTC1 and TORC2/CRTC2. Acts as a tumor suppressor and plays akey role in p53/TP53-dependent anoikis, a type of apoptosistriggered by cell detachment: required for phosphorylation ofp53/TP53 in response to loss of adhesion and is able to suppressmetastasis. Part of a sodium-sensing signaling network, probably bymediating phosphorylation of PPME1: following increases inintracellular sodium, SIK1 is activated by CaMK1 and phosphorylatesPPME1 subunit of protein phosphatase 2A (PP2A), leading todephosphorylation of sodium/potassium-transporting ATPase ATP1A1and subsequent increase activity of ATP1A1. Acts as a regulator ofmuscle cells by phosphorylating and inhibiting class II histonedeacetylases HDAC4 and HDAC5, leading to promote expression of MEF2target genes in myocytes. Also required during cardiomyogenesis byregulating the exit of cardiomyoblasts from the cell cycle viadown-regulation of CDKN1C/p57Kip2. Acts as a regulator of hepaticgluconeogenesis by phosphorylating and repressing the CREB-specificcoactivators TORC1/CRTC1 and TORC2/CRTC2, leading to inhibit CREBactivity. Also regulates hepatic lipogenesis by phosphorylating andinhibiting SREBF1.
Subunit:
Interacts with ATP1A1 (By similarity). Interacts (when phosphorylated on Thr-182 and Ser-186) with YWHAZ.
Subcellular Location:
Cytoplasm. Nucleus.
Post-translational modifications:
Phosphorylated at Thr-182 by STK11/LKB1 in complex withSTE20-related adapter-alpha (STRADA) pseudo kinase and CAB39,leading to its activation. Phosphorylation at Thr-182 promotesautophosphorylation at Ser-186, which is required for sustainedactivity. Autophosphorylation at Ser-186 is maintained bysequential phosphorylation at Thr-182 by GSK3-beta. GSK3-betacannot initiate phosphorylation at Thr-182, it can only maintainit. Phosphorylation at Ser-575 by PKA promotes translocation to thecytoplasm. Phosphorylation at Thr-322 by CaMK1 followingintracellular sodium concentration leads to activation.Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE:
Note=Defects in SIK1 may be associated with some cancers,such as breast cancers. Loss of SIK1 correlates with poor patientoutcome in breast cancers (PubMed:19622832).
Similarity:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
Database links:Entrez Gene: 150094 Human
Entrez Gene: 17691 Mouse
Entrez Gene: 59329 Rat
Omim: 605705 Human
SwissProt: P57059 Human
SwissProt: Q60670 Mouse
SwissProt: Q9R1U5 Rat
Unigene: 282113 Human
Unigene: 700423 Human
Unigene: 290941 Mouse
Unigene: 42905 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.